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        <title type="main" level="a">Assessment of the quality of marine bathing waters using different methods of percentile calculation</title>
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          <persName n="1" ref="https://orcid.org/0000-0001-6746-8693" type="ORCID">
            <forename>Diana</forename>
            <surname>Mance</surname>
            <placeName type="affiliation">University of Rijeka, Croatia</placeName>
          </persName>
          <persName n="2">
            <forename>Zrinka</forename>
            <surname>Vrček</surname>
            <placeName type="affiliation">University of Rijeka, Croatia</placeName>
          </persName>
          <persName n="3" ref="https://orcid.org/0000-0002-6731-7868" type="ORCID">
            <forename>Arijana</forename>
            <surname>Cenov</surname>
            <placeName type="affiliation">University of Rijeka, Croatia</placeName>
          </persName>
          <persName n="4" ref="https://orcid.org/0000-0001-9567-3897" type="ORCID">
            <forename>Marin</forename>
            <surname>Glad</surname>
            <placeName type="affiliation">University of Rijeka, Croatia</placeName>
          </persName>
          <persName n="5" ref="https://orcid.org/0000-0002-6206-2464" type="ORCID">
            <forename>Davor</forename>
            <surname>Mance</surname>
            <placeName type="affiliation">University of Rijeka, Croatia</placeName>
          </persName>
          <persName n="6" ref="https://orcid.org/0000-0003-0534-2547" type="ORCID">
            <forename>Darija</forename>
            <surname>Vukic Lušic</surname>
            <placeName type="affiliation">University of Rijeka, Croatia</placeName>
          </persName>
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          <resp>This is a section of <title>Tenth InternationaSymposium Monitoring of Mediterranean Coastal Areas: Problems and Measurement Techniques</title>(DOI: <idno type="DOI">10.36253/979-12-215-0556-6</idno>) by </resp>
          <name>Laura Bonora, Marcantonio Catelani, Matteo De Vincenzi, Giorgio Matteucci</name>
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        <publisher>Firenze University Press</publisher>
        <pubPlace>Florence</pubPlace>
        <date when="2024">2024</date>
        <idno type="DOI">https://doi.org/10.36253/979-12-215-0556-6.82</idno>
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          <p>Available for academic research purposes</p>
          <p>Open Access</p>
          <p>Copyright Author(s)</p>
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            <p>Content licence CC BY-NC-SA 4.0</p>
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        <p>This is original content, published for academic research purposes</p>
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      <abstract xml:lang="en">
        <p>Escherichia coli (E. coli) and intestinal enterococci (ENT) are the indicator bacteria used to test the microbiological quality of bathing water. In Croatia, the quality of bathing water is assessed by parametric method i.e. on the basis of the 90th and 95th percentile values which are calculated using the corresponding arithmetic mean and standard deviation of the logarithmic bacterial concentrations. There are also non-parametric methods for determining the value of a particular percentile, and one of them is the so-called Hazen method. In this work, we study how the different methods of percentile calculation affect the assessment of coastal bathing water quality. We have chosen a "problematic" location with frequently elevated bacterial concentrations as a test site.The results show that in the case of E. coli, the 95th percentile values calculated using the Hazen method are significantly lower than those calculated using the parametric method (Wilcoxon rank test, p &lt; 0.05), while for ENT there was no statistically significant difference. For case of annual assessment, the difference in coastal bathing water quality was statistically significant for both ENT and E. coli (McNemar test, p=0.004 and p=0.02 respectively). In the final classification, the difference in coastal bathing waters quality was statistically significant only in the case of E. coli (McNemar test, p=0.025).</p>
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        <keywords>
          <list>
            <item>microbiology</item>
            <item>bathing water quality</item>
            <item>statistical methods</item>
            <item>Hazen percentile method</item>
          </list>
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      <p>It is available online at https://doi.org/10.36253/979-12-215-0556-6.82<ref target="https://doi.org/10.36253/979-12-215-0556-6.82" /></p>
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        <listBibl>
          <head>References</head>
          <bibl n="185467">
            <bibl>Mance D., Mance D., Vukić Lušić D. (2018) - Marine pollution differentiation with stable isotopes of groundwater, Sci. J. Mar. Res. 32 (1), 80 – 87.</bibl>
            <idno type="DOI">10.31217/p.32.1.10</idno>
          </bibl>
          <bibl n="184757">
            <bibl>Mance D., Mance D., Vukić Lušić D. (2021) - Environmental and anthropogenic factors affecting coastal bathing water quality: preliminary study for Primorje-Gorski Kotar County (Croatia), Sci. J. Mar. Res. 35 (1), 57 – 68.</bibl>
            <idno type="DOI">10.31217/p.35.1.7</idno>
          </bibl>
          <bibl n="184274">
            <bibl>Mance D., Mance D., Vukić Lušić D. (2022) - Managing water commons using mediator variables to bridge the gap between environmental factors and anthropogenic pollution indicators, Ninth International Symposium “Monitoring of Mediterranean Coastal Areas: Problems and Measurement Techniques”, Livorno, Italy, 14–16 June 2022, pp. 515–524.</bibl>
            <idno type="DOI">10.36253/979-12-215-0030-1.47</idno>
          </bibl>
          <bibl n="184802">Directive 2006/7/EC of the European Parliament and of the Council of 15 February 2006 concerning the management of bathing water quality and repealing Directive 76/160/EEC. (2006) Off. J. Eur. Union 2006, L 64, 37–51.</bibl>
          <bibl n="185018">Official Gazette 73/08 (2008) - Regulation on sea bathing water quality. Croatian Ministry of Environment and Energy. https://narodne-novine.nn.hr/clanci/sluzbeni/2008_06_73_2426.html (in Croatian)</bibl>
          <bibl n="184486">Ministry of the Environment, New Zealand (2003) - Microbiological water quality guidelines for marine and freshwater recreational areas, https://environment.govt.nz/publications/microbiological-water-quality-guidelines-for-marine-and-freshwater-recreational-areas/</bibl>
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            <bibl>Jozić S., Vukić Lušić D., Ordulj M., Frlan E., Cenov A., Diković S., Kauzlarić V., Fiorido Đurković L., Stilinović Totić J., Ivšinović D., Eleršek N., Vucić A., Peroš-Pucar D., Unić Klarin B., Bujas L., Puljak T., Mamić M., Grilec D., Jadrušić M., Šolić M. (2018) - Performance characteristics of the temperature-modified ISO 9308-1 method for the enumeration of Escherichia coli in marine and inland bathing waters, Marine Pollution Bulletin, 135, 150 – 158.</bibl>
            <idno type="DOI">10.1016/j.marpolbul.2018.07.002</idno>
          </bibl>
          <bibl n="184774">ISO 7899-2 (2000) - Water quality – Detection and enumeration of intestinal enterococci – Part 2: Membrane filtration method (ISO 7899-2:2000; EN ISO 7899-2:2000). International Organization for Standardization, Geneva.</bibl>
          <bibl n="185246">Ministry of the Environment, New Zealand (2013) - Hazen percentile calculator, https://environment.govt.nz/publications/bathewatch-user-guide/hazen-percentile-calculator/</bibl>
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