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        <title type="main">Il ruolo di TAF12B e UVR3 nel ciclo circadiano dei vegetali</title>
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            <forename>Susanna</forename>
            <surname>Pollastri</surname>
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        <publisher>Firenze University Press</publisher>
        <pubPlace>Firenze</pubPlace>
        <date when="2013">2013</date>
        <idno type="DOI">https://doi.org/10.36253/978-88-6655-223-9</idno>
        <availability>
          <p>Available for academic research purposes</p>
          <p>Open Access</p>
          <p>Copyright Author(s)</p>
          <licence source="text" target="https://creativecommons.org/licenses/by-nd/3.0/legalcode">
            <p>Content licence CC BY-ND 3.0 IT</p>
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          <licence source="metadata" target="https://creativecommons.org/publicdomain/zero/1.0/legalcode">
            <p>Metadata licence CC0 1.0</p>
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        <title>Premio Tesi di Dottorato</title>
        <idno type="ISSN" subtype="print">2612-8039</idno>
        <idno type="ISSN" subtype="electronic">2612-8020</idno>
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        <bibl type="monograph">
          <edition n="1">Digital edition PDF</edition>
          <date>2013</date>
          <idno type="ISBN" subtype="electronic">978-88-6655-223-9</idno>
          <biblScope unit="page">80 pages</biblScope>
          <extent>27,63 MB</extent>
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            <p>This is original content, published in Open Access. It is also available to read for free online at <ref target="https://media.fupress.com/files/pdf/24/2447/2447_6268">https://media.fupress.com/files/pdf/24/2447/2447_6268</ref></p>
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          <date>2013</date>
          <idno type="ISBN" subtype="electronic">978-88-9273-560-6</idno>
          <availability status="free">
            <p>It is available to read for free online</p>
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        <bibl type="monograph">
          <edition n="3">Print edition</edition>
          <date>2013</date>
          <idno type="ISBN" subtype="print">978-88-6655-222-2</idno>
          <biblScope unit="page">80 pages</biblScope>
          <availability status="restricted">
            <p>It is available for online purchase at <ref target="https://books.fupress.com/isbn/9788866552239">https://books.fupress.com/isbn/9788866552239</ref></p>
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        <tag>peer-reviewed</tag>
        <rs type="FUP_policy" source="https://doi.org/10.36253/fup_best_practice">Firenze University Press Best Practice in Scholarly Publishing</rs>
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        <rs type="peer_review" resp="scientific_board" source="https://books.fupress.com/scientific-board/c/96">Premio Tesi di Dottorato 2011</rs>
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      <abstract xml:lang="en">
        <p>The book goes over the studies carried out on the circadian oscillators in plants, performed using the model plant Arabidopsis thaliana. The plant's oscillators seem to include similar components to those described for other model organisms. A bioinformatic analysis on the Arabidopsis genome found the genes TAF12b, At1g17440 and UVR3, At3g15620. Both the genes were cloned with the GFP green fluorescent protein in order to locate this protein at cell level. Furthermore, Arabidopsis SALK line plants were grown and selected, mutating the genes in question, and in parallel gene expression tests were performed on the main circadian rhythm genes in order to monitor their expression both in conditions of alternating light and dark, and in constant conditions. From the data that has emerged to date it seems that both genes are involved in the Arabidopsis's circadian rhythms.</p>
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      <abstract xml:lang="it">
        <p>Il volume ripercorre gli studi effettuati sugli oscillatori circadiani nelle piante che sono stati condotti usando la pianta modello Arabidopsis thaliana. Gli oscillatori della pianta sembrano comprendere componenti analoghi a quelli descritti per altri organismi modello. Da un'analisi bioinformatica sul genoma Arabidopsis sono stati trovati i geni TAF12b, At1g17440 e UVR3, At3g15620. Sono stati clonati entrambi i geni con la proteina fluorescente GFP (Green Flourescent Protein), al fine di individuare la localizzazione di questa proteina a livello cellulare. Inoltre sono state allevate e selezionate piantine di Arabidopsis SALK line, mutanti per i geni di interesse e in parallelo sono state condotte prove di espressione genica dei principali geni del ritmo circadiano, in modo da monitorarne l'espressione sia in condizioni di alternanza luce/buio sia in condizioni costanti. Dai dati emersi fino ad oggi sembra che entrambi i geni siano coinvolti nei ritmi circadiani di Arabidopsis.</p>
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      <p>It is available online at https://doi.org/10.36253/978-88-6655-223-9<ref target="https://doi.org/10.36253/978-88-6655-223-9" /></p>
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